Sperm test generally includes routine semen examination, seminal plasma biochemistry, immunity and trace element examination and other special examinations. 1. Routine semen examination: it can monitor the appearance, volume, liquefaction time, acidity and alkalinity, sperm concentration, sperm vitality, sperm viability, etc. of male semen to determine whether there are any abnormalities in male sperm. 2. Seminal plasma biochemistry, immunity and trace element examination: including fructose, glycosidase, acid phosphatase, seminal plasma zinc, anti-sperm antibodies and so on. 3. Other special tests: including sperm DNA integrity index, acrosomal enzymes and so on. Before collecting semen, you must stop having sex for 3~7 days, and you must not masturbate or suffer from wet dream, etc. You should also abstain from smoking and drinking, and refrain from taking medicines that have an effect on spermatogenesis. Generally take the way of masturbation to retain semen, after retaining, please send to the examination, if the patient semen abnormalities, it is recommended that patients go to the regular hospital for examination in a timely manner.