EBV is seen intracellularly. Epstein-Barr virus (EBV) is a widely spread herpesvirus.The host cells of EBV in the human body are B-lymphocytes, T-lymphocytes, epithelial cells, and myoblasts.EBV belongs to the subfamily of herpesviruses γ, which are prototypical viruses of the genus Lymphofollicular Virus. In vitro, all γ herpesviruses replicate in lymphoid cells. The EBV major outer envelope glycoprotein gp350/220 interacts with CD21 to initiate the infection process, and the initial stages of virus binding to CD21 and penetration are mediated by the major viral capsid glycoprotein gp350/220. The virus adsorbs to the surface of the B cell, causing CD21 to aggregate and “cap”, after which EBV enters the smooth membrane vesicle by cytosis. Another EBV envelope glycoprotein, gp85, then mediates the fusion of the virus with the vesicle membrane, resulting in the release of the nuclear capsid into the cytoplasm of the B cell. In summary, EBV infection is intracellular.