After iodine disinfection, the pond can usually be replenished with bacteria after about 2 to 3 days. The iodine disinfection process mainly kills the bacteria in the pond, which includes harmful bacteria and beneficial bacteria, etc. The iodine sterilization process takes 2 to 3 days. After iodine disinfection, the water body is put in a period of bacterial deficiency, when the number of bacteria is insufficient, the organic matter in the water body can not be fully decomposed, and it is not good for the circulation of the water body, in order to maintain the balance of bacteria in the water body, it is good to replenish the bacteria in about 2 to 3 days after iodine disinfection. When using iodine disinfection, the correct dose of iodine should be ensured so as not to cause imbalance in the circulation of the water body, resulting in an inappropriate dose of iodine that has a toxic effect on the aquatic products, to avoid adverse effects on the water body.