Nucleic acid testing is the detection of nucleic acid sequences of pathogens, and it is often assumed that a child’s fever does not affect nucleic acid testing. Pathogens (e.g., novel coronaviruses) contain nucleic acids, including deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Nucleic acid testing is done by collecting a specimen (e.g., nasopharyngeal specimens are often collected for novel coronaviruses), and then examining the sequence of the nucleic acids to determine whether the pathogen is infected. There are many causes of fever in children, such as acute upper respiratory tract infections, Kawasaki disease, etc. Fever is usually not considered to affect the results of the nucleic acid test, and if the nucleic acid test results are negative, the pathogen can be excluded from the test, and if they are positive, it usually indicates that the organism is infected by the pathogen being tested. Early diagnosis and treatment can be achieved by performing nucleic acid testing as early as possible. It is recommended that the patient be seen by a doctor and that the nucleic acid test be completed as prescribed by the doctor.