The process of taking urine culture includes cleaning the vulva, taking mid-stream urine, sealing it and sending it for examination. 1. Cleaning the vulva: first of all, patients should clean the vulva before taking urine culture, fully clean the vulva, foreskin, urethral opening, men mainly around the glans, to turn the foreskin to fully reveal the glans and urethral opening, women are mainly the whole vulva, usually use iodine povine and other drugs to clean. 2. Taking mid-stream urine: the first urine in the early morning should be used to ensure that the urine stays in the bladder for 6 to 8 hours. After cleansing, the patient is instructed to urinate. When urinating, the first urine is discarded and the mid-range urine is retained in a urine cup. 3. Seal: Cover the container, hands can not touch the inner mouth of the container and the lid of the container, try to ensure that there is no bacterial contamination. 4. Sent for testing: the urine specimen will be sent to the laboratory, should be done within half an hour for culture, should not be left at room temperature for too long, easy to contaminate or bacterial reproduction and thus cause false positives. In addition, if the patient has a urinary catheter or cystostomy tube, use iodine povidone to disinfect the area around the opening of the tube, clamp the tube shut for about half an hour, and then open the tube to allow the urine to flow naturally, discarding the first part of the urine, but only the urine that comes out of the middle part of the tube. For patients who need to have a urine culture, please ask at your hospital and follow the instructions of your healthcare provider or ask your healthcare provider to perform a standardized procedure.