When the seminal vesicles are empty, they will usually be back to full after 48-72 hours and the sperm count will be close to normal, there is no such thing as empty or full sperm storage. Men don’t have to worry that frequent sex will completely deplete the testes of sperm. The testes are constantly producing mature sperm and storing them in the epididymis and vas deferens, so frequent ejaculation will not empty the sperm completely.
Normally, after a man ejaculates, he empties all the semen inside the seminal vesicles and then produces new semen again. It takes time for the seminal vesicles to produce semen, and it usually takes about 48-72 hours for the semen to fill up again. If you want the semen to be a little more concentrated, it takes about 5 days for it to approach normal levels.
If sex is too frequent, the sperm count may decrease. Therefore, it is usually recommended to abstain from sex for about 3 days before a routine semen test for more accurate results. Men who have fertility requirements are advised to have intercourse more frequently around the date of ovulation of the female partner, and having intercourse once every 2-3 days will improve the chance of conception.