This test is not affected by food, but it is usually done on the second or third day of menstruation. Since the follicles in the ovaries have not yet started to develop, it can reflect the basic state of the ovaries and the hormone levels tested are more accurate. The six hormones mainly include estrogen, progesterone, androgen, luteinizing hormone, follicle stimulating hormone and lactogen, through this test, we can know what causes the patient’s irregular menstruation.