The current diagnostic methods of diabetic lower extremity arteriopathy mainly rely on angiography, CTA, MRA, etc. The disadvantage of these methods is that at the time of diagnosis, the arteries mostly already have extensive vascular sclerosis and ischemic changes in the lower extremities. In recent years, a number of studies at home and abroad have identified a variety of mechanisms associated with diabetic vasculopathy at the molecular biology level. Among them, AGEs (advanced glycation end products) have been studied more intensively. The relationship between hyperglycemia and tissue damage has been shown to be due in part to the formation and aggregation of AGEs in tissues, which is a physiological process of aging, but which accumulates earlier and more rapidly in diabetic tissues than in non-diabetic patients. The receptor for AGEs (RAGE) can now be identified by fluorescently labeled AGE proteins, the receptor protein has been isolated and purified from endothelial cell membranes, and the gene encoding RAGE has been shown to be located in the MHC region of chromosome 6 (6p 21-3). The preparation of monoclonal antibodies to AGEs with high specificity has also been reported in China for the qualitative detection of AGEs in serum and tissues. With the development of molecular imaging, the use of SPIO (Supraparamegnetic iron oxide) or USPIO (Ultrasmall The study of in vivo MR tracing with SPIO (Supraparamegnetic iron oxide) or USPIO (Ultrasmall paramagnetic iron oxide) labeled cells introduced into the living body has been reported more frequently. Since the resolution of high field strength MR can reach the cellular level, as well as the aggregation of AGEs in diabetic tissues earlier and faster than in non-diabetic patients, it is theoretically possible to use SPIO-labeled EPCs transfected with monoclonal antibody genes for AGEs to show the areas of AGEs aggregation in the arterial wall on MR. Therefore this may become one of the effective early diagnostic methods for diabetic lower extremity arterial lesions. In contrast, in vivo MR tracing using monoclonal antibodies to AGEs directly labeled with SPIO may be a more cost-effective method. Sclerosis of the vascular wall due to long-term diabetes affects not only the microcirculation and large arteries, but occasionally also large and small veins. Impaired microcirculation is one of the earliest vascular impairments in diabetes and is also one of the pathological bases for damage to other organs, including diabetic nephropathy, diabetic retinopathy, and diabetic peripheral nerve degeneration. For the monitoring of microcirculation, the research and Linchuan application of using Doppler ultrasound for detection and analysis has been carried out for many years in China and abroad. Recently, there has also been some development in the study of ultrasound contrast agents represented by SonoVue for ultrasound angiography and enhanced ultrasound perfusion imaging to evaluate microcirculatory function. Using quantitative analysis software to perform time-intensity curve analysis and calculate the peak time of microcirculatory perfusion, peak intensity variation, slope of the rising branch of the time-intensity curve, area under the curve and other indicators can distinguish normal microcirculation from damaged microcirculation. The group, Prof. Fang Ma, has been engaged in this research for a long time and has achieved many results.