Preparation for hematopoietic stem cell transplantation includes: 1. Preparation of the patient: when the tumor cell load is low, the risk of transplantation can be reduced; in addition, cardiopulmonary function, liver and kidney function tests, as well as consultations in stomatology, otorhinolaryngology, anorectology, ophthalmology, etc., to clear infections in the teeth, nasal cavity, hemorrhoids, and fundus; through blood sampling, patients are checked for viruses, bacteria, hepatitis, etc., to ensure patient safety; 2, Preparation of the donor: after the pre-match match, cardiopulmonary function, liver and kidney function, including cardiac function test, electrocardiogram, cardiac ultrasound, abdominal ultrasound and other important organ physical examination; 3. Preparation of the doctor: including ward and tool disinfection, etc.