If there is no menstruation and no pregnancy after taking progesterone, a test for estrogen can be performed, since there is no menstruation and no possibility of pregnancy after the application of progesterone, and at this time, a deficiency in the level of estrogen has to be considered. If there is menstruation after the application of hormones, it is necessary to consider that the above situation is caused by the low level of estrogen, and it is necessary to consider that the above situation is caused by the insufficiency of ovarian function. If menstruation still does not occur after the application of estrogen, it is necessary to consider whether there is a problem with the endometrium, which causes the persistent absence of menstruation as mentioned above, and hysteroscopy should be performed to find out whether there are endometrial adhesions in the uterine cavity. Therefore, if menstruation does not occur after the above situation, estrogen test can be performed to analyze the situation.