The nature of liver nodules can be clarified by imaging examination, hematological molecular markers, puncture biopsy and other examination modalities to make a definite diagnosis of liver cancer. 1. Imaging examination: imaging examination of liver cancer mainly includes ultrasonography, digital subtraction angiography, nuclear medicine, CT dynamic enhancement and MRI dynamic enhancement. For example, the blood supply of hepatocellular carcinoma is usually richer, and dynamic enhancement CT often shows “fast in and fast out”. 2. Hematological molecular markers: Hematological molecular markers of hepatocellular carcinoma are an important means to diagnose hepatocellular carcinoma. If serum AFP≥400μg/L or persistently elevated/high level, it is highly suggestive of hepatocellular carcinoma after exclusion of pregnancy, tumor of embryonic origin of the gonads, gastrointestinal tract tumor, chronic or active liver disease. 3. Puncture biopsy: preoperative puncture biopsy of hepatic lesions is generally not recommended, but for hepatic nodular lesions that lack the typical imaging features of hepatocellular carcinoma, puncture biopsy can obtain a clear pathological diagnosis. In conclusion, the definitive diagnosis of hepatocellular carcinoma requires a combination of various examination methods. Once liver nodules are found, the nature of nodules should be clarified through further examination and actively cooperate with doctors for treatment.