The detection of new crown gene fragments can be carried out mainly through molecular biology and immunological methods, which usually includes four steps, namely, nucleic acid extraction and detection, virus capture, on-line sequencing and sequence analysis.
1. Nucleic acid extraction and detection: First, the testing personnel will collect samples from nucleic acid positive cases and other cases, select samples with viral load that meet the sequencing conditions, and then prepare for gene sequencing.
2. Capture of virus: Since the new coronavirus is an RNA virus with a relatively low viral content, it is necessary to use a specialized kit to reverse transcribe the potential RNA new coronavirus into DNA fragments and capture it individually according to the characteristics of the new coronavirus, so as to find the new coronavirus.
3. Sequencing: Detectors fragment the captured virus and process the fragments to a level that can be recognized by the sequencer in preparation for subsequent sequencing.
4. Sequence analysis: the sequencer will identify and splice the new coronavirus according to different signals, and then analyze the specific data results, which will be transformed into more accurate and complete sequence data.
After the completion of this series of steps, the database can be gradually compared, thus providing help for the subsequent traceability work, and speculate the case and which outbreak is the same chain of transmission.