The accuracy of noninvasive DNA testing for twins is relatively high. Non-invasive DNA is a high-throughput gene sequencing done by fetal free gene fragments in maternal peripheral blood. According to clinical data, it has a higher accuracy rate for trisomy 13, trisomy 18, and trisomy 21 screening in twin pregnancies, which is related to the content of fetal free DNA fragments in maternal peripheral blood, and it is suitable for pregnancies between 12 and 22+6 weeks of gestation. Non-invasive DNA, as a screening tool, is not suitable for patients who have received cell therapy, allogeneic blood transfusion or organ transplantation within 1 year, and it is recommended to choose a suitable test under the guidance of obstetricians. Pregnant women should pay attention to prenatal checkups during pregnancy, actively listen to the advice of professional doctors to do a comprehensive care and health care work, pay attention to more rest, and at the same time should relax, avoid tension, anxiety, any discomfort should be timely to the hospital, the doctor to clarify the diagnosis, and cooperate with the doctor to deal with the symptoms.