There are several known subtypes of human papillomavirus (HPV), 15 of which are closely associated with the development of cervical cancer and are therefore called high-risk subtypes. High-risk subtype 16 is one of them, and 99% of cervical cancers are detected positive for high-risk subtype 16. The population is generally susceptible to HPV, but the general route of infection for high-risk subtype 16 positivity is sexual transmission or susceptibility due to low self-immunity: I. Sexual transmission: 1. Starting sex too early: having sex earlier than 16 years old, when the uterus is immature with limited immunity to the virus, it is easy to be infected with HPV and have high-risk subtype 16 positivity; 2. Having multiple sexual partners: having multiple numbers of Multiple sexual partners increase the risk of infection and even cross-infection, which may induce high-risk subtype 16 positivity; 3, early childbirth and multiple births: early age of first birth, multiple pregnancies and multiple abortions may increase the risk of disease and high-risk subtype 16 positivity; 4, inflammation: inflammation caused by sexually transmitted diseases may also stimulate the cervix for a long time and increase the risk of infection, and once infected with HPV, there may be high-risk subtype 16 positivity. subtype 16 positive situation. Second, low immunity: low immunity can lead to susceptibility to HPV, but healthy people infected with HPV, generally able to self-heal, only when the immunity is low to induce disease, high risk subtype 16 positive: 1, tired: long-term excessive exertion and anxiety, tension and other emotions, easily lead to low immunity, resulting in susceptibility to various diseases. At this time, HPV infection, prone to high-risk subtype 16 positive; 2, not exercise: people who usually exercise less prone to immune deficiency, if infected with HPV, may appear high-risk subtype 16 positive; 3, advanced age: immunity will decline with age, if infected with HPV, there may be high-risk subtype 16 positive; 4, disease: some chronic diseases that affect immunity, such as diabetes , hypertension, and AIDS, etc., the patient’s body is affected for a long time and may appear high-risk subtype 16 positive after HPV infection. Because HPV subtypes are closely related to cervical cancer, patients with positive high-risk subtype 16 should go to the hospital for screening in a timely manner. It is recommended that all women get HPV preventive vaccination as early as possible to achieve primary prevention of cervical cancer.