The following preparations are generally required: 1. Clean enema and fecal evacuation. The goal is to reduce fecal matter in the rectum, thereby producing a good sound window for prostate imaging. 2. Pre-oral prophylactic antimicrobials. Our current principle is that the patient takes an oral quinolone 30-60 minutes before the biopsy and continues treatment for 2-3 days. However, even with this approach, infectious complications cannot be completely avoided. 3, oral aspirin and other anticoagulants should be stopped for more than a week. For those with coagulation disorders or taking warfarin, prostate biopsy should not be performed until the International Normalized Ratio (INR) is corrected to below 1.5. 4. The rectal mucosa and perianal skin should be sterilized with iodophor before examination.