For women, germ cell tumors include benign tumors such as mature teratoma of the ovary and malignant tumors such as malignant teratoma and choriocarcinoma, etc. Negative AFP and β-HCG can basically exclude malignant germ cell tumors, but malignant lesions can not be completely excluded due to individual differences, and the specifics need to be followed by the doctor. AFP is the earliest plasma-binding protein appearing in the fetal period, which is produced in the yolk sac in the early embryonic period, and then produced by hepatocytes and the digestive tract. Elevated AFP is an important guideline for the diagnosis of most malignant germ cell tumors. β-HCG is a glycoprotein that is synthesized by placental syncytiotrophoblast cells during pregnancy to maintain luteal activity. Elevated serum β-HCG can be found in patients with germ cell tumors, such as choriocarcinoma and embryonal carcinoma. For women who are negative for AFP and HCG, if abnormal space-occupying lesions are found, it is recommended to go to the gynecology department of regular hospitals in time to improve CT, MRI and other examinations, and then take active treatment under the guidance of doctors after the cause is clearly identified.