Genetic testing is a very important test in recent years. The determinant of all human behavior is the genetic material in the body, called DNA. the structure and function of DNA determines the life of the body, that is, the various traits and functions of the organism. If there are problems with the genetic material, a variety of diseases can develop. Accurate monitoring of genetic material is very helpful in detecting diseases and intervening to treat them. The invention and discovery of modern molecular biology techniques and various detection technologies have led to the accurate monitoring of genetic material, as well as the accurate monitoring and treatment of various tumors. Molecular targeted therapy, as a very important therapeutic approach in recent years, is fundamentally based on accurate monitoring of genetic material and accurate sequencing to understand its details.