Preconception tests Karyotype test: available anytime. Sex hormones (FSH,LH,E2,T,PRL,DHEA-S, etc.): Blood will be drawn on the 3rd-5th day of menstruation, and you need to sit still for 15 minutes before the blood draw. Estradiol and progesterone are measured 5-7 days after ovulation. Fasting is not required. Thyroid function (FT3,FT4, TSH, TPO-Ab, etc.): no fasting is required. Blood glucose, insulin, lipids, 25-OHD, liver and kidney function: fasting is required. Blood coagulation (D-D dimer, platelet aggregation, flowmetry, homocysteine, etc.): fasting required, no recent anticoagulants or hormonal drugs, non-menstrual period. Autoimmunity (anti-cardiolipin antibody, anti-β2-GPⅠ antibody, anti-nuclear antibody, extractable nuclear antigen antibody, anti-double-stranded DNA antibody, etc.): no fasting required, exclude acute inflammatory period such as fever, upper sensation, urinary sensation, etc.; (immune indexes should be tested at least 2 times consecutively before pregnancy, each time at an interval of more than 6 weeks; rechecked at a later date after pregnancy) Vaginal discharge examination (UU/CT/white belt routine): non-menstrual period. How to measure glucose tolerance: first draw blood once on an empty stomach, drink 200-300ml of sugar water diluted with 75g of glucose powder after drawing blood, without other food, and draw blood again 2 hours later. Lupus anticoagulant, S protein, C protein, FV, antithrombin III antigen + antithrombin III activity, plasma fibrinogen (PLG), fibrinogen activation inhibitor (PAI), IgA IgM IgG T/B/NK lymphocyte subpopulation (to be carried out in our hospital, currently billed as an outpatient test in the general hematology department of Shanghai Ruijin Hospital) (to be carried out in our hospital, currently billed as an outpatient test in the general hematology department of Shanghai Ruijin Hospital ). Basal body temperature measurement method (2 menstrual cycles need to be measured): Every morning after waking up naturally, without eating or drinking, without getting out of bed or exercising, use a mercury thermometer and measure the sublingual temperature of the mouth for 3-5 minutes, and record it. Also note down the time of menstruation, the time of follicle detection, follicle size, etc. If you do not have a temperature sheet, just record it directly in a paper notebook. Follicle monitoring: For those who have regular menstruation, you can start ultrasound monitoring from the 12th day of menstruation (the first day of redness); for those who have irregular menstruation, you can start follicle monitoring from the time the urine LH test is positive, or when the leucorrhoea is increasing. If the follicle is below 15mm, retest the follicle every two days, and above 15mm, test daily until the follicle disappears. Husband: check peripheral blood chromosomes, semen routine + malformation rate + sperm fragmentation. Checked 3-5 days after ovulation.