Third-generation IVF is not required for all patients with infertility and is generally indicated for patients with genetic disorders and recurrent miscarriages. The process of third-generation IVF has four stages: comprehensive examination, egg promotion and retrieval, culture of embryos, and test transfer follow-up. 1. Comprehensive examination: Before IVF transfer, couples have to do comprehensive examination, such as routine blood test for hepatitis B, syphilis, HIV, hepatitis C, hepatitis A, etc., to determine whether they have the conditions to undergo third-generation IVF. 2. Ovulation promotion and retrieval: the female partner has to take ovulation promotion injections so as to promote the discharge of eggs. The doctor will take out the very ripe eggs under the guidance of ultrasound. 3. Cultivation and testing of embryos: After the eggs and sperm are removed, fertilization and embryo cultivation will take place. Through the three-generation IVF, the embryos need to be tested, so they are cultured to blastocysts. Patients need to go to the hospital to learn about the blastocyst culture and the number of embryos to be sent for testing, and after the test results come out, the time of freezing and transferring will be arranged according to the specific situation. 4. Transfer Follow-up: After the transfer, the mother-to-be carries out HCG supplementation, while the doctor will carry out luteal support according to the mother-to-be’s physical condition. Serum HCG test will be conducted 12-14 days after embryo transfer to determine whether there is pregnancy. Serum HCG is measured again 21 days after embryo transfer to see how the embryo is developing. Transvaginal ultrasound at 30 days after embryo transfer to determine if there is an intrauterine pregnancy with or without fetal heartbeat.