The significance of using estradiol after transfer is to safeguard the endometrium’s tolerance to embryos and facilitate embryo implantation. In patients who use estradiol, usually for IVF, embryos are transferred after egg retrieval or frozen embryos are thawed and transferred, and many patients are using alternative cycles of embryo transfer, so estradiol is used. Estradiol should not be discontinued without authorization. Failure to use estradiol may result in failure of embryo implantation, or miscarriage after embryo implantation, or cessation of development. Therefore, estradiol after transfer, its significance is very important, do not stop using it without permission, but use it strictly as prescribed by your doctor, who will tell you to gradually reduce the dosage of estradiol until it is stopped. Therefore, the use of estradiol after transplantation is very important and should be used strictly according to the doctor’s prescription.