Syphilis spirochete antibodies include spirochete-specific and spirochete-nonspecific antibodies, which can be detected by blood or cerebrospinal fluid. Syphilis spirochete is a kind of antibody produced by the body after infection with syphilis, including specific antibody and non-specific antibody, the detection of syphilis spirochete antibody is of great significance for the diagnosis and prevention of syphilis. The syphilis serum test can determine whether there is syphilis antibody in the serum; in addition, the cerebrospinal fluid examination is mainly to rule out syphilis. There are many methods that can help the body to determine syphilis antibody, such as rapid plasma reactivity test can determine the non-specific antibody of syphilis spirochete, while the microhemagglutination test of syphilis spirochete antibody, fluorescent spirochete antibody uptake test and so on can determine the specific antibody of syphilis spirochete. The diagnosis of syphilis needs to be combined with clinical symptoms and signs as well as relevant examination results, and individuals should receive scientific treatment as soon as possible if the diagnosis is confirmed.