Anti-ENA antibody spectrum is one of the main tests used to diagnose rheumatic immune system diseases, mainly including anti-SSA antibody, anti-SSB antibody, anti-Sm antibody, anti-Jo-1 antibody, anti-r-RNP antibody, anti-U1-RNP antibody. ENA is a general term for extractable nuclear antigens, and the anti-ENA antibody spectrum is often used clinically as an important indicator for the diagnosis of rheumatic immune system diseases. For example, if anti-SSA and anti-SSB antibodies are positive, it is necessary to be alert to dry syndrome, and part of the positivity can be seen in systemic lupus erythematosus; anti-Sm antibody positivity suggests systemic lupus erythematosus; anti-Jo-1 antibody positivity suggests myositis and dermatomyositis; anti-r-RNP antibody and anti-U1-RNP antibody positivity needs to be alert to mixed connective tissue disease, and so on. Although the anti-ENA antibody profile is an important indicator of disease diagnosis, a positive antibody profile does not necessarily confirm the diagnosis of a particular disease, but needs to be combined with the patient’s symptoms and other results to confirm the diagnosis. It is recommended that patients with a positive anti-ENA antibody profile go to the hospital for prompt treatment under the guidance of a medical professional.