Myoglobin has a role in transporting and storing oxygen within myocytes, and diffuses out of myocytes into the circulation in the event of myocardial damage. Myoglobin is a small molecule pigmented protein, consisting of a combination of bead protein and orthoferrin, which can show reversible binding to oxygen. Therefore, myoglobin is capable of transporting and storing and distributing oxygen for the human body. In addition, the measurement of myoglobin levels can assist to some extent in the diagnosis of certain diseases, such as the degree of damage to cardio-skeletal muscle. Since myoglobin is widely distributed in cardiac and skeletal muscles, an increase in the concentration of myoglobin in the blood may indicate damage to cardiac and skeletal muscles. Therefore, if the concentration of myoglobin in the blood is higher than normal, it is necessary to be vigilant for acute myocardial infarction, ischemic heart disease, cardiogenic shock, myotonic dystrophy, etc., which needs to be determined by a professional doctor in a timely manner.