High-risk human papillomavirus quantification <500 is within the reference range and is mostly a normal result. High-risk human papillomavirus, or HPV, mainly includes HPV subtypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68, of which HPV 16 and 18 have the highest probability of inducing cancerous lesions, such as cervical and penile cancers, which is a serious danger to human health. High-risk HPV-DNA test is an indicator to determine whether there is a large number of virus replication at present, and also an indicator to understand the effect of treatment. Most hospitals adopt real-time fluorescence quantitative PCR testing method, HPV-DNA reference value result <500 can be diagnosed as negative, i.e., not infected or cured. Different hospitals adopt different instruments and equipment, different testing methods and raw materials, different PCR settings of the internal reference, HPV-DNA reference value has some differences, it is recommended to analyze the results with the reference value range after the laboratory test. If you suspect that you are infected with HPV, it is recommended that you should seek medical attention in a timely manner and follow the doctor's instructions for formal examination and treatment.