Semen analysis is an important test in male medicine and is essential for reproductive disorders or for those who may have abnormal sperm quality due to other diseases, as well as for pre-fertility testing. The examination can provide insight into the quality of sperm, the quantity and function of sperm, and also the functional status of the man’s reproductive system and the related endocrine organs. To make semen analysis valuable, it is first necessary to make the results of semen analysis authentic, objective and scientific, and this depends on reasonable and correct operation. First of all, the length of abstinence can have a great impact on the overall semen analysis results, which is an important prerequisite for the authenticity and objectivity of semen analysis results; although studies have shown that the frequency of ejaculation does not correlate with sperm density and sperm viability, it correlates with the volume of semen (volume responding to the secretion capacity of the glands) and the total number of sperm (responding to the spermatogenic status of the testes and the patency of the post-testicular ductal system). Clinical requirements generally call for 3-7 days of abstinence (including masturbation, sexual intercourse, and seminal emission) prior to specimen collection. At the same time, semen specimens should be collected with the same abstinence time, such as abstinence for 5 days before the first semen collection, and preferably the same abstinence for 5 days thereafter. Secondly, semen analysis and examination should be performed in the best physical condition recently to avoid systemic diseases and bad habits that can affect sperm quality in the short term to affect the diagnosis of infertility. Thirdly, masturbation method is the best for collection. If there is difficulty, a sperm extractor can be used for collection, and interruption of intercourse is prohibited because this method will lose a part of the semen before which the sperm density is the highest. It is not possible to collect semen with the usual latex or plastic condoms because this may affect the quality of the sperm. Fourth, the integrity of the semen specimen collection is very important, that is, the ejaculated semen should be collected completely in the specimen box without spilling. Fifth, the temperature of the semen specimen should be maintained at 25°C-35°C after collection for storage in the laboratory or during transfer. If the transport time is too long (more than 2 hours), or if there is leakage in the container holding semen, such specimens cannot be analyzed for semen. Sixth, specimens should be collected in a warm, comfortable and relaxed environment to ensure a smooth sperm collection process. Seventh, semen analysis is affected by a variety of factors, and judgments cannot be made on the basis of only one semen result, and should generally be repeated at intervals of 1-2 weeks.