The new crown O gene stands for open reading frame, which is an open reading frame gene in the genome of novel coronaviruses, and the new crown N gene stands for nucleocapsid protein, which is a nucleocapsid protein gene in the genome of novel coronaviruses. Both are target genes for nucleic acid testing of new coronaviruses. Usually, both O and N genes are positive, suggesting that a person is infected with the virus. If one of the O or N genes is positive, it is necessary to take samples again for real-time fluorescence RT-PCR to determine whether the person is infected or not. The ninth edition of the “new coronavirus pneumonia diagnosis and treatment program” pointed out that two consecutive intervals of 24 hours of nucleic acid O gene and N gene are greater than 35, in order to leave the square cabin. Normally, you need to pay attention to wear a good mask, pay attention to hand hygiene, avoid going to crowded areas, and actively cooperate with nucleic acid testing. If you feel unwell, consult a doctor in time. Tips] The “new coronary pneumonia, new coronary virus pneumonia” mentioned in this article has been renamed to “new coronary virus infection” on December 26, 2022, as announced by the National Health Commission.