Only through the Ki67 index of 30% can’t judge whether the tumor is moderately differentiated or not, it is necessary to combine with immunohistochemistry to judge the degree of differentiation of the tumor. Ki67 is an immunohistochemical index, through which clinicians can read the proliferation ability of tumor tissue cells, the larger the Ki67 value is, the stronger the proliferation ability of the tumor tissue, the higher the degree of malignancy, which can help clinicians to initially choose the treatment (the stronger the proliferation ability of the tumor, the more sensitive it is to radiation and chemotherapy). The gold standard for determining the degree of differentiation of tumor tissues is pathological biopsy, in which tumor tissue sections are examined under the microscope to observe their cellular anisotropy and cell nuclear division. The degree of differentiation of tumor cells needs to be judged by professional doctors in the Department of Pathology, and patients should read and understand the degree of differentiation of the tumor under the guidance of professional doctors.