The Kim’s method is accurate for the determination of serum glutamine aminotransferase. The Kim’s method, along with the Rai’s method and the modified Rai’s method, are chemical colorimetric assays and are commonly used for the detection of glutamine transaminase. Glutaminase is found in the liver, heart, and skeletal muscle. When liver cells or certain tissues are damaged or necrotic, alanine aminotransferase values are elevated. Elevated alanine aminotransferase can be seen in conditions such as myocarditis, hepatitis, cholecystitis, liver damage from medications, heavy alcohol consumption, and strenuous exercise. Often need to be combined with other tests to clarify, such as viral hepatitis also need to be combined with viral series; such as viral myocarditis can have abnormal blood routine; such as cholecystitis need to be combined with liver and gallbladder ultrasound. If there is an abnormality in the enzyme alanine aminotransferase, it is recommended to consult a doctor to clarify whether there is a related disease.