A 16G puncture needle is a puncture needle with an inner diameter of 1.19mm at the tip of the needle, and puncture needles with a needle diameter greater than 1.0mm belong to coarse needles, so 16G puncture needles belong to coarse needles. Clinically, there are more choices about biopsy needles, the most commonly used is the cutting biopsy needle, such as Tru-cut needle, the size of its tube diameter varies, commonly used 14G, 16G and 18G, etc.; the puncture doctor usually chooses the large diameter biopsy needles, thinking that the larger the diameter of the tube, the more tissues will be extracted from the puncture, and the higher the diagnostic accuracy may be. Puncture biopsy is to locate the suspicious lesion, insert the hollow thick needle into it, take out 3~4 strips of tissue specimens with a diameter of about 1mm and a length of about 1~2cm to be sent for pathological biopsy, and after puncture, it is necessary to press the puncture site for about 20 minutes, if there is any panic, dizziness, bleeding from the wound, etc., it should be informed to the doctor so that the appropriate treatment can be done in time.