The correct way to take a urine specimen for urine culture varies between men and women. For many patients with urinary tract infections, it is necessary to take a urine specimen for urine culture to determine whether there are pathogenic bacteria in the urine, and after clarifying the causative organisms, the doctor will be able to target the causative organisms to carry out sensitive drug treatment. 1. Male: male patients need to do perineal disinfection before taking urine specimens, after disinfection, male patients open the foreskin, the glans and the urethral orifice is completely exposed to normal urination, in the process of urination, keep the mid-range of the urine for urine culture examination. 2. Female: female patients need to do perineal disinfection before urine specimen, after disinfection, female patients separate the vaginal opening to expose the urethral opening for normal urination, in the process of urination to retain the middle part of the urine for urine culture examination. Through the correct method of urine specimens for urine culture examination results are more meaningful, because the initial part of the urine is often mixed with a small number of bacteria, only to take the middle part of the urine, urine culture of pathogenic bacteria results are more accurate, clinical reference significance. Patients should follow the doctor’s instructions to standardize the urine specimen for urine culture examination, so as not to affect the test results, if the test results are abnormal, please consult a doctor in time for treatment.