IVF II, mainly for men with oligospermia and weak spermatozoa, is a means of assisting pregnancy, resulting in infertility, with most success rates around 40%. The specific probability of success is also related to the patient’s condition, age, and the physical condition of the female partner. The older the male is, the lower the relative success rate, and the lower the quality as well as the quantity of the sperm, the lower the relative success rate. The success rate of IVF II will be improved if the female partner is in good health and does not have menstrual cycle disorders or recurrent miscarriages. Moreover, the younger the woman is, the better the ovarian function is, and the higher the relative success rate is, some of which can reach 60%. Before the second generation of IVF, both men and women need to undergo a series of tests, and according to the results of the tests, the appropriate IVF method will be adopted to improve the success rate of IVF.