Progesterone assay means measuring the level of progesterone in a woman’s body, which helps in clinical diagnosis and treatment of diseases. Progesterone is a kind of progesterone, produced by the ovarian corpus luteum after ovulation, such as patients with menstrual disorders, the measurement of the level of progesterone in the body can assist in determining whether the female ovary ovulation, assisting in the clinical diagnosis of abnormal uterine bleeding for ovulation, or anovulatory abnormal uterine bleeding, thereby guiding the clinical use of medication. If the patient is in the early stage of pregnancy, a sufficient amount of progesterone is conducive to the growth and development of the embryo. Measuring progesterone levels at this time, and providing timely supplementation when the level of progesterone is too low, can, to a certain extent, avoid miscarriage and other adverse pregnancy outcomes. If the result of progesterone measurement is abnormal, standardized treatment should be carried out under the guidance of professional physicians to avoid delaying the condition and leading to adverse consequences.