The standardized use of New Coronavirus 2019-nCoV Nucleic Acid Test Kit, including the process of sample collection, preservation and transfer, sample nucleic acid extraction and detection, and interpretation of results, needs to be operated by professional medical personnel. 1. Sample collection: Use pharyngeal swab or anal swab to collect the specimen in the throat or anus, and place it in the specimen preservation solution for preservation. 2. Sample preservation: the specimen tubes need to be properly preserved in accordance with the specified temperature and humidity to avoid abnormalities in temperature, humidity and storage time, which may affect the accuracy of the test results, and attention should also be paid to the preservation process to ensure that the specimens are not contaminated, and that they do not contaminate other items and the environment. 3. Transfer: to have specimen preservation equipment by the special vehicle for specimen transfer, transportation. Pay attention to keep the sealing and integrity of the specimen preservation tubes in the process of transfer to avoid breakage. 4. Specimen testing: new coronavirus transmission capacity, high risk factor, the laboratory for sample testing, should have personal protection should be biosafety level 3 laboratory level. Each operator must have professional skills and PCR test qualification. The entire operation process to prevent aerosol contamination on the risk of infection of personnel. 5. Interpretation of the results: After the correct test, the test results should be correctly interpreted by experienced test personnel, who should be able to identify false negatives and false positives. In order to prevent the spread of the virus, nucleic acid testing must be performed by medical professionals and should be well protected.