It is possible to take probiotics to regulate after gallbladder removal. Because the gallbladder has the role of concentrating and storing bile, which can help to digest food, the removal of the gallbladder will affect the human body’s digestive system. Probiotics is through the regulation of intestinal flora, and then assist in the treatment of constipation and diarrhea. Probiotics can secrete digestive enzymes, reduce the depth of small intestinal crypts, increase the surface area of the small intestine, and promote the absorption of intestinal nutrients. Probiotics can also improve the body’s immunity, improve some of the patient’s allergic symptoms, decompose the body’s toxic carcinogenic substances, and also have a good auxiliary therapeutic effect on the adverse consequences caused by taking antibiotics. It is recommended that patients should strictly control their diet in the first month after surgery, low-fat diet, eat less and more meals, and try to eat easy-to-digest food. The scientific and rational use of probiotics can really benefit the improvement of human digestive system.