Clostridium tetani is found mainly in contaminated soil and feces. Generally speaking, Clostridium tetani is highly resistant and is found in nature in the form of spores, especially on soil, feces, and rusty iron. At the same time, the spores are highly resistant to boiling for 40-50 minutes and can survive for decades in the soil. When the human body wounds, especially the presence of narrow and deep puncture wounds, trauma necrotic tissue is more, is the human body infected with tetanus clostridium difficile disease morbidity risk factors. This is because deeper wounds and more localized tissue damage induce ischemia and hypoxia, and Clostridium tetani, which is an anaerobic bacterium, will proliferate in the right environment. And release a large amount of neurotoxin, leading to uncontrolled muscle spasms and death in the body. Therefore, when there is a narrow and deep puncture wound or wound contact with soil, etc., must be timely inoculated with tetanus vaccine, to achieve timely and effective post-exposure prophylaxis, to avoid the occurrence of tetanus a serious threat to life and health safety.