Glycocholic acid is a chemical substance secreted by the liver, which can reflect the function of the liver and its hepatobiliary material circulation, and is important for the diagnosis of hepatobiliary system diseases. Serum glycocholic acid is synthesized in the liver by combining bile acid and glycine, and its normal metabolic pathway is the intestinal-hepatic cycle, and the content of glycocholic acid in normal people is 0.4-2.98 mg/L. If the content of glycocholic acid in the routine blood test is increased, it suggests that the liver cells of the patient are damaged or cholestasis occurs, so glycocholic acid is of great significance to diagnosis of diseases of the liver and biliary system. In addition, cirrhosis, hepatocellular carcinoma, and acute and chronic hepatitis may also show elevated values of glycocholic acid. However, the presence of elevated glycocholic acid in women during pregnancy is generally not a cause for concern, as it is normal for glycocholic acid to be elevated by 30 to 60% during pregnancy. It is recommended that patients other than pregnant women with elevated glycolic acid go to the hospital promptly.