Methotrexate may elevate the cardiac enzyme profile. Methotrexate belongs to the folic acid class of antitumor drugs, which is mainly used in the chemotherapy of breast cancer, malignant hyperemesis gravidarum, leukemia and other diseases, in addition to the treatment of autoimmune diseases, such as systemic lupus erythematosus, dermatomyositis and so on. The use of this drug may cause liver function damage, which may cause the indicators of alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase and other indicators to be elevated. The cardiac enzyme profile includes lactate dehydrogenase, ghrelin, phosphocreatine kinase, phosphocreatine kinase isoenzyme, and ghrelin, and so on, and therefore the cardiac enzyme profile may be elevated after taking methotrexate. The drug may also cause cough, shortness of breath, myelosuppression, headache and other discomforts. It is contraindicated for people with severe hepatic and renal insufficiency, and for those who are allergic to the components of the drug; in the process of using the drug, it is necessary to pay attention to monitoring the blood routine, coagulation function, hepatic and renal function, and so on. The drug should be used under the guidance of a doctor, avoid self-medication to avoid adverse consequences.