EBV can generally be detected by antigen detection, antibody detection and isolation and culture of the virus. 1. Antigen detection: EBV DNA in the specimen can be detected by in situ nucleic acid hybridization test or PCR to prove the existence of EBV infection, and immunofluorescence can also be used to detect EBV antigen in cells. 2. Antibody test: The presence or absence of EBV lgG antibody or lgM antibody in the body can be detected to assist in the diagnosis of EBV infection. 3. Virus isolation and culture: this method is the gold standard, but it takes a long time and is not commonly used in the clinic, if the virus is isolated, the diagnosis can be confirmed. In addition, when the body is infected with EBV, the white blood cells in the blood routine may be normal or increased, and abnormal lymphocytes may appear, which can be used as an auxiliary reference item. When infected with EBV, it should be actively treated under the guidance of a doctor.