Non-invasive DNA testing does not require fasting. Non-invasive DNA test is a clinically mature prenatal screening method, through the extraction of peripheral blood of pregnant women, isolation of fetal free DNA in the peripheral blood, high-throughput sequencing, combined with specific genetic information analysis methods to assess the risk of fetal chromosomal disorders. Fasting or not will not affect the expression of fetal free DNA and the screening results, so fasting is not required for non-invasive DNA testing. Non-invasive DNA testing is mainly used to screen for trisomy 21, trisomy 18, and trisomy 13, and is suitable for pregnant women who are at a critical risk for Down’s syndrome and those who have missed the optimal screening time for Down’s syndrome. If the non-invasive DNA test indicates a high risk, further confirmatory testing, i.e. amniocentesis, is required to clarify whether the fetus has any chromosomal abnormalities; pregnant women with low-risk results from the non-invasive DNA test do not need to undergo amniocentesis.