Biotherapy is a mode of tumor treatment that goes hand in hand with surgery, radiotherapy and chemotherapy, and has the characteristics of safety and little side effects. Biotherapy is applicable to a variety of solid tumors, including: renal cancer, malignant melanoma, lung cancer, liver cancer, colorectal cancer, gastric cancer, breast cancer, prostate cancer, bladder cancer, leukemia, and squamous carcinoma of head and neck, etc., which can be applied, and the treatments with surgery, chemotherapy, radiotherapy, minimally invasive therapy, etc. It can be used in combination with surgery, chemotherapy, radiotherapy, minimally invasive therapy and other treatments. Li Yan, Department of Oncology and Chemotherapy, Shandong Qianfoshan Hospital Currently, our department carries out tumor biotherapy projects: 1. Autologous cytokine-induced killer cell (CIK) therapy: it is the first choice of new generation of anti-tumor super-immunotherapy, and the method of large-capacity expansion of this kind of cells has a good prospect of clinical application in the treatment of tumors. It has better efficacy in the clinical treatment of early, middle and advanced malignant solid tumors, and better efficacy in the treatment of surgical resection, intervention, radio frequency, argon helium knife and other treatments at the same time or after the treatment. Part of the patients with tumors who are not suitable for surgery, intervention or other treatments for the time being can also undergo CIK cell therapy first in order to improve the immune function of the organism. 2. Autologous dendritic cell (DC) therapy: dendritic cells are the cells with the strongest antigen-presenting function in the known organism, and after loaded with tumor antigens, they can stimulate T lymphocytes to differentiate into cytotoxic T lymphocytes with anti-tumor activity, and carry out specific killing of tumor cells, so they are the first choice for active immunotherapy. This treatment method has better clinical effect in the treatment of malignant solid tumors. Tumor biotherapy adopts a variety of treatment modes such as combined minimally invasive surgery, chemotherapy, radiotherapy, targeted therapy or used alone to control advanced tumors or maintenance therapy. In lung cancer, hepatocellular carcinoma, renal cancer, prostate cancer and other tumors, a significant difference in 1-year PFS, OS has been achieved, and some patients have reached CR. our department has established a perfect quality control system for cell culture, biotherapy cases and laboratory archives, and has applied for a number of Shandong Province scientific and technological development plan projects through the joint application with multiple specialties such as thoracic surgery, urology, breast surgery, gynecology, geriatrics, gastroenterology, etc., and through the relevant relevant natural fund projects. As the main committee unit of Shandong Biomedical Engineering Society’s Bio-directed Therapy Committee, our department has promoted biotherapy in the province. Over the past 6 years, tumor cellular immunotherapy in the province has made great development and achieved better clinical efficacy. Biological therapy has been increasingly emphasized by clinicians, patients and their families.