Prostatitis can also result in a positive nitrite test, which correlates with the specific type of inflammation, and the presence of a positive test is indicative of a gram-negative bacterial infection. Prostatitis includes both aseptic and bacterial prostatitis. Clinically, bacterial prostatitis, such as Escherichia coli infection, can lead to a positive nitrite assay and needs to be treated as a bacterial infection. In most cases, a drug sensitivity test is needed, and then antibiotics are regularly applied for control. A positive test is mainly a guide for diagnosis and treatment. Prostatitis is accompanied by obvious symptoms such as abdominal cramps, abnormal urination and even fever, which require timely medical observation, diagnosis of the specific condition through various tests, and standardized treatment under the guidance of the clinician to avoid delays in the condition.