ASCUS is the abbreviation of atypical squamous cells of undetermined significance, it is a frequent finding in the liquid-based cytology results of cervical cancer screening, which is commonly known as the squamous cells seen, suggesting the possibility of lesions. It is recommended to further improve colposcopy and cervical biopsy pathology examination in hospital, if inflammatory stimulation, HPV virus infection, senile vaginitis and other diseases are identified, they can be treated according to Inflammation: Inflammatory reaction in the cervix increases leukocytes and will result in atypical squamous cells of unknown significance during cytology, and patients are advised to take oral antibiotic therapy. During treatment, unprotected sex should be prohibited and the patient’s sexual partners within the last 60 days should also be evaluated, tested and treated. 2. Aged vaginitis: The patient’s ovarian function declines, the glycogen content in the epithelial cells decreases, the pH in the vagina rises, the local resistance decreases and pathogenic bacteria are prone to invade and multiply causing inflammation, which in turn results in atypical squamous cells of undetermined significance. Patients can use metronidazole suppositories vaginally for the cause under the guidance of the doctor. 3. HPV infection: it will suggest atypical squamous cells of unknown significance during the examination. It is recommended to check cervical HPV typing to understand whether there is high-risk HPV infection, and if combined with high-risk HPV infection, it is recommended to perform colposcopy, cervical biopsy under the instruction of colposcopy, and pathological examination. Patients are also advised to be treated according to the HPV typing of the infection. For cervical intraepithelial neoplasia grade I-II, dynamic observation or cervical electrocautery may be performed, and for neoplasia grade II-III, cervical conization therapy is recommended.