Puncture biopsy is an important way used in medicine to detect bone or soft tissue tumors in the patient’s body for pathological diagnosis, and the operation process is different for different parts of the human body. However, puncture biopsy should generally do the following points: First, before performing puncture biopsy, it must be careful and prudent to make a good puncture plan to avoid serious complications. Second, when performing the puncture procedure, it must be ensured that it is performed in a sterile environment. Third, the results of the biopsy must be accurate to ensure that the surgeon develops the correct surgical or treatment plan. Fourth, the patient’s tissue standards enable the pathologist to make a diagnosis, and if the diagnosis is not made, details of the patient’s imaging and clinical symptoms need to be provided. Fifth, if the hospital where the patient consults is not able to carry out the treatment of tumor, the patient should be transferred to the hospital that can carry out the treatment of tumor in time.