The significance of deoxyribonucleic acid sequencing can check whether the fetus is suffering from chromosomal disorders, and it is recommended that serologic screening reveals that people at high risk of fetal autosomal aneuploidy need to undergo deoxyribonucleic acid sequencing tests. Deoxyribonucleic acid sequencing test is a way of prenatal diagnosis of pregnant women, deoxyribonucleic acid sequencing is mainly through the extraction of fetal free deoxyribonucleic acid in the peripheral blood of pregnant women, and then through the sequencing of the examination, and also need to sequence the results of the biological analysis, which can get the genetic information of the fetus. The test can determine whether the fetus has any of the three major chromosomal disorders, such as Down’s syndrome, Edward’s syndrome, and Patau syndrome. Pregnant women are advised to seek medical attention for DNA sequencing if they find that their fetus has a risk of aneuploidy between the high-risk cut-off value and 1/1000 for common chromosomes during serologic screening. Deoxyribonucleic acid sequencing is more accurate in diagnosing fetal chromosomal genetic disorders, and it is recommended that pregnant women at high risk should seek medical attention in a timely manner.