Liver function tests require fasting. Liver function tests are commonly used laboratory tests in clinical practice, and the liver function pentameter usually includes five items: alanine aminotransferase, glutamine aminotransferase, bilirubin, albumin, and globulin. However, in clinical work, sometimes renal function tests and ion tests, called biochemical tests, are also performed. In the biochemical tests include blood glucose, blood lipids, kidney function and other liver function indicators such as alkaline phosphatase, bilirubin and direct bilirubin. Liver function tests do not include Hepatitis B tests, i.e., two half pairs, and Hepatitis B penta. Generally Hepatitis B Penta is only found in immunology tests. Hepatitis B Penta includes Hepatitis B Surface Antigen, Hepatitis B Surface Antibody, E Antigen, E Antibody and Core Antibody. Based on the test results, it is possible to determine whether the patient has hepatitis B virus infection. It is important to make sure that you abstain from eating and drinking before any biochemical test so that the test can be accurate.