24-hour urine protein quantification is the collection of all urine excreted in a 24-hour period. The “24-hour urine volume” in 24-hour urine protein quantification means that the first urine in the morning is expelled, and the second urine is kept from the beginning. The time of the first urine is recorded for 24 hours until the next day. Put all the urine from each of these 24 hours into a container, mix it well, and then take 50 ml of it to the hospital for testing. The results of the urine protein quantification test can accurately measure the amount of protein excreted in the urine. In order to accurately measure the 24-hour urine protein quantification, the urine in the bladder should be emptied and discarded before 8:00 a.m., and a timer should be started to store all the urine excreted for 24 hours in a container (including the urine that is allowed to be relieved at 8:00 a.m. the next day) and send it all for testing. If you have a bowel movement within the 24 hours, you should first collect the urine and then remove it. Small volumes of urine should also not be missed. If the urine volume is not collected completely, the calculation of urine protein volume will not be accurate. Before the test, we should measure the total amount of urine in a measuring cup, then stir it well, take out a small cup to determine the amount of protein per 50-100 ml, and then calculate it according to the actual amount of urine, and we can calculate the amount of protein for 24 hours. In short, to accurately measure the 24-hour urine protein amount, the entire day’s urine volume must be accurately collected and the test portion must be a mixture of the total urine volume in order to be representative.