Petechiae on the conjunctiva are one of the clinical manifestations of sepsis. The clinical onset of sepsis is rapid, and the rash takes the form of petechiae, urticaria, pustular rash, and scarlet fever-like rash with petechiae on the conjunctiva. Gram-negative coccus sepsis can cause petechiae on the conjunctiva. The diagnosis of sepsis can be determined by the patient’s clinical symptoms and relevant examination findings. The possibility of sepsis should be considered in any patient with acute fever and markedly increased leukocytes and neutrophils without acute infection confined to a particular system. History questioning and detailed physical examination are of some significance in assisting the diagnosis with presumed etiology. Anyone with a recent skin infection or trauma, especially with extruded sores. Or there are infected lesions of the urinary tract, biliary tract, respiratory tract, etc. Or a variety of local infections despite effective antibacterial drug treatment, but the body temperature can not be controlled, should be highly suspected of the possibility of sepsis, in addition, some tests should be performed to assist in the diagnosis. The clinical diagnosis of sepsis can be basically established if the patient develops rash, hepatosplenomegaly, migratory abscess, etc. during the course of the disease. A detailed clinical physical examination often reveals the primary lesion or invasion route, and the type of pathogenic bacteria can be inferred from the site and nature of the lesion. Further testing should be performed after obtaining a positive blood culture, which often leads to the discovery of the primary lesion and facilitates radical treatment. A positive laboratory test by blood culture (and bone marrow culture) is the basis for a definitive diagnosis of sepsis.