The diagnosis of tuberculosis relies on a combination of clinical symptoms, physical examination, and laboratory tests to confirm the diagnosis. First of all, there may be symptoms such as cough, coughing sputum, hemoptysis, low fever, night sweats and menstrual disorders. At the same time, the diagnosis can be made on the basis of the following tests: 1. Chest X-ray examination shows typical imaging manifestations, and chest X-ray examination can not only detect tuberculosis at an early stage, but also determine the location, nature and extent of the lesions, understand the onset of disease and be used to judge the effect of treatment. Positive PPD test is one of the evidence of TB infection. 3. Positive TB antibody test is also helpful for diagnosis. 4. Polymerase chain reaction (PCR), less specific, has the advantage of sensitivity up to 98%-100%. 5. T-SPOT.TB test has better sensitivity and specificity than PPD test for TB examination. 6. Sputum smear test. Sputum TB test is simple and easy to perform with high accuracy, and the detection of TB bacilli in sputum can confirm the diagnosis of TB disease. Generally, three sputum specimens are examined at the first visit, namely nocturnal sputum, early morning sputum and immediate sputum. Although it is the “golden indicator” for the diagnosis of tuberculosis, the low positive rate is a shortcoming.7. Sputum tuberculosis culture, which has high reliability and can be used for mycobacterial identification and drug sensitivity testing, takes 6-8 weeks and is limited in application.8. Fiberoptic bronchoscopy: It can directly observe or indirectly determine bronchial and pulmonary lesions, and has the functions of biopsy, lavage, video recording and taking pictures of the trachea. Thoracoscopy and mediastinoscopy: both can be used to observe enlarged lymph nodes in the chest and mediastinum, and biopsies can be taken out for diagnosis and differential diagnosis.10 Lymph node aspiration biopsy is meaningful for the diagnosis of lymphatic tuberculosis.11 Percutaneous lung aspiration pathological tissue examination is helpful for the differential diagnosis of pulmonary tuberculosis. Through the above methods, the majority of tuberculosis can be diagnosed clearly.