H. pylori culture is difficult. Helicobacter pylori is a gram-negative bacterium. Firstly, its growth conditions are harsh and the culture process is complicated, secondly, it is easy to harbor other stray bacteria during culture, and furthermore, the culture of this bacterium requires excellent laboratory conditions and experienced technicians, so the cultivation of Helicobacter pylori is relatively difficult. Because H. pylori is so difficult to culture, it is rarely used for clinical testing, but rather for scientific research to guide precise treatment. For example, some patients with H. pylori infection have special conditions and have failed many treatments, so they need to culture H. pylori and then do drug resistance testing (culture the bacteria and then add antibiotics in vitro to see which type of antibiotics they are sensitive to). Culturing H. pylori is done by specialized doctors and technicians, so patients do not need to worry about whether it is difficult or not, but if necessary, patients need to actively cooperate with the doctor to do the preparations before the culture.