Nucleic acids can be done twice in 24 hours without significant effect on the body, but two nucleic acid tests in 24 hours are not routinely required. Currently, when screening for new coronavirus infections in medium- to high-risk areas, there are occasions when two or more nucleic acid tests are performed in one day, and performing two nucleic acid tests within 24 hours can help to improve screening efficiency and minimize the occurrence of false-negative results. Two nucleic acid tests within 24 hours are generally not associated with adverse effects and should not be a cause for concern. If nausea occurs after the test, gargle with warm water or salt water to relieve symptoms. The result of one nucleic acid test can only represent whether or not the new coronavirus is detected at the time of this nucleic acid sampling. Two nucleic acid tests within 24 hours for high-risk groups can make the result more reliable, but it is generally not required to do it twice within 24 hours.